Anti-carcinogenic Activity of Ruellia Tuberosa L. (acanthaceae) Leaf Extract on Hepatoma Cell Line & Increased Superoxide Dismutase Activity on Macrophage Cell Lysate

Objective: The current study was planned to investigate the anti-carcinogenic & anti-oxidant property of Ruellia tuberosa L. (Acanthaceae) methanolic leaf extract (MERTL) on HepG2 cell line & RAW 264.7 murine macrophage cell lysate respectively. Methods: The potent cytotoxic activity of MERTL has been studied by cell growth inhibition study & MTT assay on metabolically active HepG2 cells. 1% Agarose gel electrophoresis, detection of apoptosis & cell cycle arrest by Flow cytometric analysis have been performed to determine the proportion & stages of cellular apoptosis on HepG2 cells. In anti-oxidant study, Superoxide dismutase activity study of MERTL & its various fractions has been performed on RAW 264.7 cell lysate. Results: Cytotoxicity study of MERTL has been confirmed by MTT assay & the IC50 value is calculated to be 54.95μg/ml on HepG2 cell line. Agarose gel electrophoresis study showed fragmented DNA in the form of ladder. Flow cytometric analysis showed appreciable number of cells in early apoptotic stage. The cells are getting arrested mostly in G0/G1 phase & also in G2/M phase of cell cycle. Anti-oxidant property of MERTL & its fractions has been confirmed by increased Super oxide dismutase activity. Conclusion: Ruellia tuberosa L. (Acanthaceae) leaf extract possess potent apoptogenic activity on HepG2 cell line & the same showed notable antioxidant activity on RAW 264.7 cell lysate.